Preparation of Overnight Cultures and Bioassay Plates
2:20
Inoculating Bioassay Plates with the Test Organism
2:58
Preparation and Inoculation of the Target Organism
5:02
Results: Co-culture Interaction Assays Uncover Siderophore-mediated Inhibition of Coagulase-negative staphylococci by Corynebacterium propinquum
6:06
Conclusion
Transcription
Our protocol provides a high throughput method for screening a large number of pairwise microbial interactions in tandem to identify interactions of interest which can aid microbiome research and antimicrobial discovery. This method is readily app
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.