Two-dimensional cell cultures do not adequately mimic in vivo tumor growth. To improve, 3D procedures have been developed using spheroids. Our 3D glioblastoma spheroid-based assay is particularly well-suited to investigate brain tumor invasion int
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Here, we describe an easy-to-use invasion assay for glioblastoma. This assay is suitable for glioblastoma stem-like cells. A Fiji macro for easy quantification of invasion, migration, and proliferation is also described.