Blastocoele Extraction and Analysis of Tgf β Cleavage Products
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Results: Analysis of Cleaved BMP Ligands in Xenopus laevis Blastocoele Fluid
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Conclusion
Transcription
This protocol can be used to study the process by which a wider range of segregated precursor proteins, including TGF-beta family members, are converted to active proteins following proteolytic cleavage. The main advantage of this protocol is that
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When Transforming Growth Factor ß family precursor proteins are ectopically expressed in Xenopus laevis embryos, they dimerize, get cleaved and are secreted into the blastocoele, which begins at the late blastula to early gastrula stage. We describe a method for aspirating cleavage products from the blastocoele cavity for immunoblot analysis.