Isolate Single-Cell Populations from Dental Follicle
2:52
Results: Emergence of Epithelial Cells
3:13
Conclusion
Transcription
This protocol demonstrate an enhanced method for procuring human dental epithelial cells. It is difficult to identify the aggregated cell pellet during the procedures. Be careful when you handle supernatant to prevent the loss cell populations.
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The dental follicle contains an epithelial population and mesenchymal cells. The epithelial population was selected from the heterogeneous dental follicle cell population by providing a distinct culture medium. Epithelial cells survived and formed colonies in a serum-free medium.