Incubation and Windowing of Chick Eggs to Prepare for Membrane Dissection
3:50
Microdissections of the Extraembryonic Membranes
5:08
Corneal Wounding
6:49
Results: Scarless Tissue Regeneration in Embryonic Wounded Chick Corneas
8:35
Conclusion
Transcription
Damage to the adult corneal stroma leads to a rapid wound healing response that compromises one's vision due to opaque scar tissue formation. This protocol generates wounds in embryonic chick corneas which unlike adults, can heal without a scar. G
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The present protocol demonstrates the different steps involved in wounding the cornea of an embryonic chick in ovo. The regenerating or fully restored corneas can be analyzed for regenerative potential using various cellular and molecular techniques following the wounding procedure.