Preparation of Culture Medium and Isolation of Uterus and Removal of Embryos
3:59
Setting up the Culture System
5:20
Evaluating Development after Whole Embryo Culture
6:10
Representative Embryo Cultures
7:08
Conclusion
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.