Whole Cell Electrophysiology of EC Cells from Primary Culture
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Results: Culture Results and Throughput of Whole-cell Electrophysiology
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Conclusion
Trascrizione
This method can help answer key questions in enteric neuroscience and gastrointestinal physiology, such as, what are the mechanisms of enterochromaffin cell excitability and response to luminal stimuli, and what are the mechanisms of gastrointesti
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Enterochromaffin (EC) cells comprise a small subset of gastrointestinal epithelial cells. EC cells are electrically excitable and release serotonin, yet difficulties in culturing and identifying EC cells have limited physiological studies. The method presented here establishes a primary culture model amenable to examination of single EC cells by electrophysiology.