1. 기부자 운영 <ol><li> 기증 쥐 24 시간 (물 / 혈당 솔루션을 무료로 이용하실 수)에 금식 보관해야합니다. </li><li> Isoflurane 흡입 마취를 유도하기 위해 표준 분무기에 2 %로 시작하고 개복술을 수행 한 후 1 % 줄일 수 있습니다. 진정 작용을 확인 발가락 핀치을 수행합니다. </li><li> 복부를 면도하고 피부 시험을 치루 3 회 (Kodan)로 청소하십시오. 그런 다음 진통제의 피하 투여 후 중간 절개를 수행합니다. </li><li> 이식은 생리 불린 거즈에 싸서되면 췌장 및 Q-팁 (6x 확대와 함께 수술 현미경)을 부드럽게 오름 콜론 사이의 생리 adhesions를 구분합니다. </li><li> Ligate와 7-0 실크를 사용하여 ileocecal &amp; 중간 &amp; 오른쪽 복통 혈관을 나눕니다. 오름 콜론은 장간막 정맥의 오른쪽 (SMV)에 퍼져 후, ileocecal 오른쪽 중간 잘록 창자 선박은 7-0 관계를 사용하여 결합 및 분할에 대한 식별 할 수 있습니다. </li><li> 전체 SMV는 정리와 노출되어 있도록 위쪽으로 배를 철회. 철회를위한 모기 겸자를 사용하십시오. 클램프는 필요에 따라 모양으로 형성 plasticine 질량에 의해 개최됩니다. </li><li> Ligate와 SMV에서 오는 pancreatico - 십이지장 정맥을 나눕니다. 췌장 조직 이식에서 제거 할 수 있습니다 전에 SMV에서 발생하는 모든 작은 pancreatico - 십이지장 정맥은 신중하게, 확인 7-0 실크로 출혈도 잡았와 구분해야합니다. </li><li> Ligate와 SMV과 복부 대동맥 사이 lymphatics를 포함하여 손실 결합 조직을 나눕니다. 여전히 복부의 오른쪽에있는 그라프로는 SMV 및 복부 대동맥에 액세스 할 사이 lymphatics 등의 결합 조직을 잃게됩니다. 이 결합 조직은 7-0 실크를 사용하여 출혈도 잡았와 장 이식의 수술 lymphorrhea을 피하기 위해 나눌 수 있어야합니다. </li><li> Ligate와 오른쪽 신장 동맥을 나눕니다. 결합 조직이 분열 한 후, 오른쪽 신장 동맥은 accessibl된다전자와는 출혈도 잡았와 7-0 실크를 사용하여 구분됩니다. </li><li> 체계적으로 음경 정맥을 통해 헤파린 IV 200 단위를 사용하여 쥐를 heparinise. </li><li> 한계 동맥을 Ligate하고 duodeno-jejunal 교차로에서와 터미널 회장에서 작은 창자를 나눕니다. </li><li> 대동맥은 SMA의 출처에 proximally 출혈도 잡았 있습니다. 포털 정맥은 지라 정맥과 합류에 가로로되어 있습니다. 그런 다음 이식은 대동맥 세그먼트와 SMA 자사의 혈관 작은 꽃자루로 구성된을 수확하고 있습니다. </li></ol> 2. Backtable 절차 <ol><li> 대동맥 도관을 통해 4에 위스콘신 솔루션 (UW)의 대학 ° C의 3 ML을 사용하여 이식을 Perfuse와 4 ° C (루멘 관개는 필수입니다)에서 Uro​​-Nebacetin N 솔루션의 30 ML과 jejunal 끝에서 장 내강을 관개 . </li></ol> 즉시 이식을 추출 후 대동맥 도관은 3 ML 차가운 UW 솔루션과 재관류에 사용됩니다. 에서이 들어, 20 G IV 카테터10 ML의 주사기가 사용됩니다. perfusate은 나누어 포털 정맥에서 자유롭게 밖으로 흘러하기 위해 관찰해야합니다. Nebacetin있는 장 관개를 들어, 50 ML의 주사기가 사용됩니다. <ol start="2"><li> 받는 사람의 준비 기간 동안 4 ° C에서 UW 솔루션에 이식을 저장합니다. </li></ol> 3. 받는 운영 <ol><li> 받는 사람 쥐 24 시간 (물 / 혈당 솔루션을 무료로 이용하실 수)에 금식 보관해야합니다. </li><li> Isoflurane 흡입 마취를 유도하기 위해 표준 분무기에 2 %로 시작하고 개복술을 수행 한 후 1 % 줄일 수 있습니다. 진정 작용을 확인 발가락 핀치을 수행합니다. </li><li> 복부를 면도하고 피부 시험을 치루 3 회 (Kodan)로 청소하십시오. 그런 다음 intraoperative 진통제에 대한 Carprofen 5 밀리그램 / kg SC의 피하 투여 후 중간 절개를 수행합니다. </li><li> 정상적인 생리 불린 거즈에받는 장을 정리하고받는 사람의 가슴에 놓습니다. </li><li> Q-팁과 퉁명스럽게 retroperitoneum를 열고 abdomi 노출단지 필요한 경우 다운 엉덩 혈관의 수준에 신장 혈관, 아래 NAL 대동맥과 아래 대정맥은 microscissors을 사용합니다. 혈액 손실을 방지하기 위해 7-0 실크를 사용하여 대동맥과 대정맥에서 작은 허리와 척추 tribuaries을 Ligate. (이 작업을 수행하려면 16X에 수술 현미경 줌을 변경할 수 있습니다.) </li><li> distally microvessel 클립을 사용하여 왼쪽 신장 혈관 proximally와 엉덩 분기 위 아래에있는 대동맥과 IVC를 건너서 - 클램프. 하나의 클램프를 동시에 혈관 클램프에 distally뿐만 아니라 proximally 사용됩니다. anteriorly microknife를 사용하여 모두 혈관을 절개하고 나머지 피를 씻어. </li><li> 연속 10-0 Prolene의 봉합을 사용하여 그라프 트 대동맥 세그먼트과받는 사람의 infrarenal 대동맥 사이의 엔드 - 투 - 측 문합 부위를 만들기 .. 처음에는 이식은 낮은 숙박 봉합의 동맥 문합하고 매듭 묶느라 뒷면 벽 바늘을 수행 할 수있는 복부의 오른쪽 (12시 위치 쥐의 머리)에 배치됩니다. 일실내, 이식은 문합의 전면 벽을 노출하고 봉합 할 수있는 복부 (8시와 9시에 위치 쥐의 머리)의 왼쪽으로 설정되어 있습니다. </li><li> 그라프 트 포털 정맥과받는 사람의 IVC 사이의 엔드 - 투 - 측 문합은 10-0 Prolene을 사용하여 봉합을 실행하여 마찬가지로 수행됩니다. 쥐와 함께 누워있는 옆으로는 (9시 위치에 머리), 이식은 복부의 왼쪽에 위치하며 낮은 숙박 봉합이 배치됩니다. 문합은 선박 내부에서 다시 벽으로 시작됩니다. 낮은 숙박 봉합이 연결된 후 전면 벽 바늘은 외부에서 수행 할 수 있습니다. </li><li> 상단 클램프 다음 먼저 말초 클램프를 제거합니다. 모든 anastomotic의 출혈은 Q-팁을 사용하여 직접 압력에 의해 제어됩니다. 이식은 동등하고 신속하게 reperfusion를 확인한다. </li><li> 창 자간 막 혈관의 결합 후 전체받는 사람의 작은 창자를 잘라내 다. 수상자는 프로로부터 2-3 센티미터 보존 소계 enterectomy을 받다ximal 공장 및 말초 회장의 1cm. </li><li> 근위 (jejuno-jejunostomy)과 말초 (ileo-ileostomy) 엔드 - 투 6-0 Monocryl을 중단 한 레이어 봉합을 사용하여 장 문합을 종료합니다.이 장의 연속성을 복원 약 16 봉합은 문합을 완료하기 위해 필요합니다. </li><li> 깨끗한 때까지 정상적인 생리와 복막 캐비티를 관개. 유체 교체 정상적인 생리 intraperitoneally 2 ML을 관리 할 수​​ 있습니다. 그런 다음 3-0 근육 레이어 Vicryl 플러스 지속적으로 피부 봉합과 연속 봉합을 사용하여 복부를 닫습니다. </li><li> 수술 기간에서 쥐 그런 다음 표준 쥐 수유와 물 광고 libitum에 다시 시작 또 다른 24 시간에 (물과 포도당 솔루션에 액세스 할 수있는) 금식 보관해야합니다. carprofen있는 진통제는 3 일 (이하 복용량을 참조) 관리해야합니다. </li></ol>

<ol>
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관심 없음 충돌이 선언 없습니다.

쥐의 장 이식 모델 1970ies 5로 일찍 설명되어 있지만 최근 고용 모델의 대부분은 다른 기술이 13을 사용하여 heterotopic 장 이식을 포함한다. 일반적으로 heterotopic 절차를 쉽게 microsurgical 기법과 평가를위한 이식을보다 쉽게​​ 접근 할 수있는 장점을 가지고 있지만, heterotopic 장 이식이 계정에 이식 된 작은 창자의 여러 상호 작용과 수축 활동 등의 기능 측면을 복용하지의 가장 큰 단...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td><td> 코멘트 (선택 사항) </td></tr><tr><td> 위스콘신 대학 (UW) 솔루션 (ViaSpan) </td><td> 브리스톨 - 마이어스 Squibb </td><td></td><td></td></tr><tr><td> Uro-Nebacetin N 솔루션 </td><td> Nycomed </td><td> 6967855 </td><td></td></tr><tr><td> 암피실린 </td><td> Ratiopharm </td><td></td><td></td></tr><tr><td> Carprofen (Rimadyl) </td><td> 화이자 </td><td></td><td></td></tr><tr><td> Prolene 10-0 unresorbable 봉합 </td><td> Ethicon </td><td></td><td></td></tr><tr><td> Monocryl 6-0 resorbable 봉합 </td><td> Ethicon </td><td></td><td></td></tr><tr><td> Vicryl 3-0 resorbable 봉합 </td><td> Ethicon </td><td></td><td></td></tr><tr><td> IV 카테터 G 20 1.1x33 mm </td><td> 브라운 </td><td></td><td></td></tr><tr><td> IV 카테터 G 22 0.9x25 mm </td><td> 브라운 </td><td></td><td></td></tr><tr><td> Kodan 피부 준비 </td><td> Schülke </td><td></td><td></td></tr><tr><td> NaCl 0.9 % 주입 솔루션 </td><td> 브라운 </td><td></td><td></td></tr><tr><td> 구부러진 집게 작은 </td><td> FineScienceTools </td><td> 11009-13 </td><td></td></tr><tr><td> 마이크로 포셉가 휘어 </td><td> AESCULAP </td><td> BD 333 </td><td></td></tr><tr><td> 마이크로 포셉가 휘어 </td><td> AESCULAP </td><td> FD281R </td><td></td></tr><tr><td> 마이크로는 곧바로 1 포셉 </td><td> WPI </td><td> 5 </td><td></td></tr><tr><td> 마이크로는 곧바로 두 집게 </td><td> WPI </td><td> 이 </td><td></td></tr><tr><td> 마이크로 니들 홀더 </td><td> WPI </td> &lt;TD&gt; 14,081 </td><td></td></tr><tr><td> 마이크로 가위 </td><td> FineScienceTools </td><td> 15006-09 </td><td></td></tr><tr><td> 마이크로 메스 </td><td> 마니 </td><td> 안과 칼 </td><td></td></tr><tr><td> 마이크로 클램프 </td><td> AESCULAP </td><td> FB329R </td><td></td></tr></tbody></table>

orthotopic small bowel transplantation in rats

작은 창자 이식은 비경 영양 짧은 창자 증후군과 실패 (돌이킬 수없는 장 실패) 환자에 대한 임상 승인 옵션이되고 있습니다. 전문 센터에서 수술을 향상과 생활 1,3의 높은 품질을 제공하는 동시에 관리 전략은 우수한 단기 및 중간 기간 환자와 이식 생존으로 이어 왔습니다. 다른 고체 기관의보다 일반적인 이식과 달리 (예 : 심장, 간) 장 이식에 의해 유도 그라프 트 기능과 immunologic의 변경의 여러 기본 메커니즘은 완전히 알려져 6,7 없습니다. 급성 거부, 패혈증 및 만성 이식 실패의 에피소드는 아직 덜 유리한 장기 성과에 기여하고 잠재적으로 이러한 이식의 혜택을받을 수 집 비경 영양에 대한 환자의 증가에도 불구하고 절차의보다 광범위한 고용을 방해 주요 장애물입니다. 소장을 포함여객 leucocytes의 큰 숫자가 일반적으로 내장 관련된 림프 시스템 (갈트) 장 이식의 높은 immunogenity 이유의이되는 부분의 일부라고. 이식 점막 무결성 (거절의 예를 들어) 손상되면 존재 속에서 많은 commensals과 병원균의 근접 패혈증 에피소드의 심각도를 설명합니다. 안정적이고 실현 가능한 동물 모델에서 생성 된 더 많은 장 및 multiorgan 이식 데이터의 필드를 이동하는 것은 필요합니다. 모델은 여기에 한 번 표준화 된 방식으로 설립 신뢰성과 타당성을 모두 결합하여 장 이식에 의해 실행되는 기본 복잡한 분자, 세포 및 기능 메커니즘에 중요한 통찰력을 제시 할 수 있습니다 제공됩니다. 우리는 성공적으로 우리의 실험실 8-11에서 5 년 이상 동안 사용 설명 절차를 수정했습니다. 주피터 동영상 기반 형식은 복잡한 proce을 설명 할 때 특히 유용합니다dure 및 장 이식을 조사 orthotopic 쥐 모델을 구축 할 계획 그룹에 대한 초기 함정을 피하십시오.

일반 수술 과정 이식 동물은 약 ​​1 시간에 대한 열 램프 아래의 절차에서 신속하게 복구해야합니다. 저체온은 수술 사망률의 주요 원인이며, 신중하게 내부 및 postoperatively 피해야한다. Intraoperative 유체 손실 2.5 ML 정상적인 생리의 SC 주입 플러스 2.5 ML 포도당 5퍼센트 첫 24 시간마다 8 시간으로 대체해야합니다. 쥐도 postoperatively 첫​​ 24 시간에 포도당 용액 (또는 겔 다이어?...

Watch this Scientific Journal Video about Orthotopic Small Bowel Transplantation in Rats at JoVE.com

Orthotopic Small Bowel Transplantation in Rats

작은 창자 이식은 되돌릴 장 실패 환자에 대한 인정 치료 옵션이되었습니다. 쥐의 orthotopic 작은 창자 이식 우리의 실험 모델은 장 이식을 복잡하게 기본 immunologic과 염증 프로세스를 처리 할 수​​있는 신뢰할 수있는 도구로 제공하고 있습니다.

쥐 Orthotopic 작은 창자 이식

Small  bowel transplantation has become an accepted clinical option for patients with  short gut syndrome and failure of parenteral nutrition ...

orthotopic-small-bowel-transplantation-in-rats

Research

JoVE Journal

Medicine

14.1K 조회수.  University of Bonn, Germany. 작은 창자 이식은 되돌릴 장 실패 환자에 대한 인정 치료 옵션이되었습니다. 쥐의 orthotopic 작은 창자 이식 우리의 실험 모델은 장 이식을 복잡하게 기본 immunologic과 염증 프로세스를 처리 할 수​​있는 신뢰할 수있는 도구로 제공하고 있습니다.

비디오: Orthotopic Small Bowel Transplantation in Rats

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and standardized not only for the study of allo-rejection but also between syngeneic strains for examining mechanisms of ischemia-reperfusion injury after lung transplantation. Although the application of the rat and other large animal model 2 contributed significantly to the elucidation of these studies, the scope of those investigations is limited by the scarcity of knockout and transgenic rats. Due to no effective therapies for obliterative bronchiolitis, the leading cause of death in lung transplant patients, there has been an intensive search for pre-clinical models that replicate obliterative bronchiolitis. The tracheal allograft model is the most widely used and may reproduce some of the histopathologic features of obliterative bronchiolitis 3. However, the lack of an intact vasculature with no connection to the recipient's conducting airways, and incomplete pathologic features of obliterative bronchiolitis limit the utility of this model 4. Unlike transplantation of other solid organs, vascularized mouse lung transplants have only recently been reported by Okazaki and colleagues for the first time in 2007 5. Applying the basic principles of the rat lung transplant, our lab initiated the obliterative bronchiolitis model using minor histoincompatible antigen murine orthotopic single-left lung transplants which allows the further study of obliterative bronchiolitis immunopathogenesis6.

Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.

Orthotopic 폐 이식의 Murine 모델에 Obliterative Bronchiolitis의 개발

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and ...

연구

의학

Orthotopic Liver Transplantation in Rats

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat liver transplantation in 1979 by Kamada et al.3, this model has remained the gold standard despite various proposed alternative techniques4. Nevertheless, its broader use is limited by its steep learning curve5.
In this video paper, we show a simple and easy-to-establish revision of Kamada's two-cuff technique. The suprahepatic vena cava anastomosis is performed manually with a running suture, and the vena porta and infrahepatic vena cava anastomoses are performed utilizing a quick-linker cuff system6. Manufacturing the quick-linker kit is shown in a separate video paper.

We present an easy-to-establish revision of the classical two-cuff technique for orthotopic liver transplantation in rat.

쥐에게 Orthotopic 간 이식

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. This is largely true, but there are a number of important principles that can reduce the difficulty of these procedures and enhance reproducibility. Orthotopic aortic transplantation is an excellent procedure in which to learn these principles because it involves only two end-to-end anastomoses, but requires good suturing technique and handling of the vessels for consistent success. This procedure begins with the procurement of a length of abdominal aorta from a donor animal, followed by division of the native aorta in the recipient. The procured aorta is then placed between the divided ends of the recipient aorta and sutured into place using end-to-end anastomoses. To accomplish this objective successfully requires a high degree of concentration, good tools, a steady hand, and an appreciation of how easily the vasculature of a mouse can be damaged, resulting in thrombosis. Learning these important principles is what occupies most of the beginner's time when learning microsurgery in small rodents. Throughout this protocol, we refer to these important points. This model can be used to study vascular disease in a variety of different experimental systems1-8. In the context shown here, it is most often used for the study of post-transplant vascular disease, a common long-term complication of solid organ transplantation in which intimal hyperplasia occurs within the allograft. The primary advantage of the model is that it facilitates quantitative morphometric analyses and the transplanted vessel lies contiguous to the endogenous vessel, which can serve as an additional control9. The technique shown here is most often used for mice weighing 18-25 grams. We have accumulated most of our experience using the C57BL/6J, BALB/cJ, and C3H/HeJ strains.

We describe a technique in which a section of the abdominal aorta from a mouse is transplanted orthotopically to just below the renal arteries in an allogeneic or syngeneic recipient. This technique can be useful in studies in which transplantation of large arteries of uniform size is deemed advantageous.

혈관 질병의 연구에 대한 마우스의 Orthotopic 대동맥 이식

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Orthotopic Hind Limb Transplantation in the Mouse

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and translational research in the field of transplantation medicine. A vast array of reagents is available exclusively in this setting, including mono- and polyclonal antibodies for both diagnostic and interventional applications. In addition, a vast number of genotyped, inbred, transgenic, and knock out strains allow detailed investigation of the individual contributions of humoral and cellular components to the complex interplay of an immune response and make the mouse the gold standard for immunological research. 
Vascularized Composite Allotransplantation (VCA) delineates a novel field of transplantation using allografts to replace "like with like" in patients suffering traumatic or congenital tissue loss. This surgical methodological protocol shows the use of a non-suture cuff technique for super-microvascular anastomosis in an orthotopic mouse hind limb transplantation model. The model specifically allows for comparison between established paradigms in solid organ transplantation with a novel form of transplants consisting of various different tissue components. Uniquely, this model allows for the transplantation of a viable vascularized bone marrow compartment and niche that have the potential to exert a beneficial effect on the balance of immune acceptance and rejection. This technique provides a tool to investigate alloantigen recognition and allograft rejection and acceptance, as well as enables the pursuit of functional nerve regeneration studies to further advance this novel field of transplantation.

This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in&#160;vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).

마우스에서 소성을 뒷다리 이식

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats

Hepatocellular carcinoma (HCC) is a disease with limited treatment options and poor prognosis. In recent years, oncolytic virotherapies have proven themselves to be potentially powerful tools to fight malignancy. Due to the unique dual blood supply in the liver, it is possible to apply therapies locally to orthotopic liver tumors, which are predominantly fed by arterial blood flow. We have previously demonstrated that hepatic arterial delivery of oncolytic viruses results in safe and efficient transduction efficiency of multifocal HCC lesions, resulting in significant prolongation of survival in immune competent rats. This procedure closely mimics the application of transarterial embolization in patients, which is the standard palliative care provided to many HCC patients. The ability to administer tumor therapies through the hepatic artery in rats allows for a highly sophisticated preclinical model for evaluating novel viral vectors under development. Here we describe the detailed protocol for microdissection of the hepatic artery for infusion of oncolytic virus vectors to treat orthotopic HCC.

Oncolytic virotherapies are under development as novel therapeutics for the treatment of hepatocellular carcinoma (HCC). Here we describe a method for locoregional therapy of HCC via hepatic arterial administration of oncolytic virus.

쥐에서 동소 이식 간세포 암의 국소 치료에 대한 종양 살상 바이러스의 간동맥 관리

Hepatocellular carcinoma (HCC) is a disease with limited treatment options and poor prognosis.  In recent years, oncolytic virotherapies have proven ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

정형외 심장 이식의 전 임상 돼지 모델

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of renal replacement therapy. Over the past few decades, the rat kidney transplantation model has been used to study the immunological phenomena of rejection and tolerance. This model has become an indispensable tool to test new immunomodulatory pharmaceuticals and regimens prior to proceeding with expensive preclinical large animal studies.
This protocol provides a detailed overview of how to reliably perform orthotopic kidney transplantation in rats. This protocol includes three distinctive steps that increase the probability of success: perfusion of the donor kidney by flushing through the portal vein and the use of a cuff system to anastomose the renal veins and ureters, thereby decreasing cold and warm ischemia times. Using this technique, we have achieved survival rates beyond 6 months with normal serum creatinine in animals with syngeneic or tolerant kidney transplants. Depending on the aim of the study, this model can be modified by pre- or posttransplant treatments to study the acute, chronic, cellular, or antibody-mediated rejection. It is a reproducible, reliable, and cost-effective animal model to study different aspects of kidney transplantation.

The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

직교 쥐 신장 이식: 새롭고 단순화 된 외과 접근

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model.

The goal of this study is to modify the rat orthotopic liver transplant model to better represent human liver transplantation and improve recipient survival. The presented method reestablishes hepatic arterial inflow by connecting the donor liver&#39;s common hepatic artery to the recipient liver&#39;s proper hepatic artery.

직교 간 이식의 쥐 모형에 있는 동맥 재연결 후에 감소된 합병증

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for kidney transplantation (KT). Even though, the use of allografts from extended criteria donors (ECD) could partially ease the shortage of organ donors, ECD organs carry a potentially higher risk for inferior outcomes and postoperative complications. Dynamic organ preservation techniques, modulation of ischemia-reperfusion and preservation injury, and allograft therapies are in the spotlight of scientific interest in an effort to improve allograft utilization and patient outcomes in KT.
Preclinical animal experiments are playing an essential role in translational research, especially in the medical device and drug development. The major advantage of the porcine orthotopic auto-transplantation model over ex vivo or small animal studies lies within the surgical-anatomical and physiological similarities to the clinical setting. This allows the investigation of new therapeutic methods and techniques and ensures a facilitated clinical translation of the findings. This protocol provides a comprehensive and problem-oriented description of the porcine orthotopic kidney auto-transplantation model, using a preservation time of 24 hours and telemetry monitoring. The combination of sophisticated surgical techniques with highly standardized and state-of-the-art methods of anesthesia, animal housing, perioperative follow up, and monitoring ensure the reproducibility and success of this model.

Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.

24시간 장기 보존 및 지속적인 원격 분석을 사용하여 돼지 모델에서 직소 신장 자동 이식

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for ...

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. However, lung transplantation is limited by a shortage of available donor organs. As such, there is high waitlist mortality. Ex situ lung perfusion (ESLP) has increased donor lung utilization rates in some centers by 15%-20%. ESLP has been applied as a method to assess and recondition marginal donor lungs and has demonstrated acceptable short- and long-term outcomes following transplantation of extended criteria donor (ECD) lungs. Large animal (in vivo) transplantation models are required to validate ongoing in vitro research findings. Anatomic and physiologic differences between humans and pigs pose significant technical and anesthetic challenges. An easily reproducible transplant model would permit the&#160;in vivo&#160;validation of current ESLP strategies and the preclinical evaluation of various interventions designed to improve donor lung function. This protocol describes a porcine model of orthotopic left lung allotransplantation. This includes anesthetic and surgical techniques, a customized surgical checklist, troubleshooting, modifications, and the benefits and limitations of the approach.

This protocol describes a juvenile porcine model of orthotopic left lung allotransplantation designed for use with ESLP research. Focus is made on anesthetic and surgical techniques, as well as critical steps and troubleshooting.

ESLP를 위한 어린 돼지 모델에서의 좌측 폐 정형외과적 이식

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. ...