June 10th, 2022
•This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.
Related Videos
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Isolation of Murine Valve Endothelial Cells
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Isolation of Murine Coronary Vascular Smooth Muscle Cells
Identification and Dissection of Diverse Mouse Adipose Depots
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Copyright © 2024 MyJoVE Corporation. 판권 소유