Generation of Human Brain Organoids for Mitochondrial Disease Modeling

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6.0K Views

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08:09 min

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June 21st, 2021

DOI :

10.3791/62756-v

June 21st, 2021

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Stephanie Le¹, Laura Petersilie², Gizem Inak¹^,⁴, Carmen Menacho-Pando¹, Karl W. Kafitz², Agnieszka Rybak-Wolf³, Nikolaus Rajewsky³, Christine R. Rose², Alessandro Prigione¹^,⁵

¹Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty, Heinrich Heine University, ²Institute of Neurobiology, Heinrich Heine University, ³Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrueck Center for Molecular Medicine (MDC), ⁴Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, ⁵Max Delbrueck Center for Molecular Medicine (MDC)

Podsumowanie

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Brain Organoids

Mitochondrial Disease

Human IPSCs

Cortical Differentiation Medium

ROCK Inhibitor

Neurospheres

Cell Culture Protocol

Interventional Targets

Feeder free Conditions

Tissue Culture Incubator

Cell Suspension

Seeding Medium

WNT Catenin Inhibitor

SB 431542

Rozdziały w tym wideo

0:05

Introduction

0:44

Generation of Brain Organoids

5:31

Immunostaining of Brain Organoids

6:07

Results: Visualization and Bioenergetic Profiling of Brain Organoids Generated from iPSCs

7:34

Conclusion

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