Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

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3.1K Views

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10:43 min

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December 23rd, 2022

DOI :

10.3791/64676-v

December 23rd, 2022

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Samuel Flashner*¹, Cecilia Martin*¹^,², Norihiro Matsuura¹, Masataka Shimonosono¹, Yasuto Tomita¹, Masaki Morimoto¹, Ogoegbunam Okolo¹, Victoria X. Yu¹^,³, Anuraag S. Parikh¹^,³, Andres J. P. Klein-Szanto⁴, Kelley Yan¹^,², Joel T. Gabre¹^,⁵, Chao Lu¹^,⁶, Fatemeh Momen-Heravi¹^,⁷, Anil K. Rustgi¹^,⁵, Hiroshi Nakagawa¹^,²^,⁵

¹Herbert Irving Comprehensive Cancer Center, Columbia University, ²Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, ³Department of Otolaryngology, Head and Neck Surgery, Columbia University, ⁴Histopathology Facility, Fox Chase Cancer Center, ⁵Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, ⁶Department of Genetics and Development, Columbia University, ⁷Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University

Podsumowanie

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Esophageal Squamous Cell Carcinoma

Genetic Heterogeneity

Therapeutic Strategies

Functional Annotation

Mutagen induced Tumors

Organoid Preparation

Paraffin embedding Procedure

Cervical Trachea

Thoracic Trachea

Abdominal Esophagus

Dissection Procedures

Surgical Techniques

Rozdziały w tym wideo

0:04

Introduction

1:28

Animal Dissection and Organ Collection

4:39

Isolation of Keratinocytes from the Dissected Mouse Tissue

6:07

Seeding of the Initial Cell Suspension

6:54

Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)

7:52

Preparation of Organoids for Paraffin Embedding

8:48

Results: Characterization of the Organoids

10:03

Conclusion

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