Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

1:01

Genome‐Wide Substitution Mutagenesis of JFH1 Using Error‐Prone PCR

2:46

Estimation of the Proportion of Mutations in ep‐PCR Products (Mutant Libraries)

6:08

Viral RNA Transfection of the Huh7.5 Cell Line

7:57

Quantification of Virus Titers

11:59

Drug‐Resistant Viral Variant Selection

13:33

Results: Integration of ep‐PCR and Virus Reverse Genetics to Generate HCV Mutants

15:17

Conclusion