Lentivirus-Renilla-Luciferin-Red Fluorescent Protein (LV-Rluc-RFP; RR) and LV-Rluc-RFP-Herpes Simplex Virus Truncated Thymidine Kinase (HSV-ttk) (LV-Rluc-RFP-TTK; RT) Packaging and Production
3:38
LV-RR and LV-RRT Lentiviral Transduction for Gene Expression in 4T1 Cells
4:37
Drug Screening and LV-RR and LV-RRT-Transduced 4T1 Cell Identification
5:18
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Firefly Luciferase (Fluc)-Knockin (KI) Mice and Tumor-Bearing Mouse Model
6:50
Dual Bioluminescence Tumor (Rluc) and Angiogenesis (Fluc) Imaging
7:58
Results: Representative In Situ Tumor Growth and Angiogenesis Visualization and Analysis
9:52
Conclusion
Transkript
Angiogenesis play important role in tumor metastasis and progression. In this protocol, we will use dual bioluminescence imaging method to monitor the dynamic tumor progression and angiogenesis in a mouse model of breast cancer. In this model, we
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This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.