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Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters

DOI :

10.3791/51795-v

July 8th, 2014

July 8th, 2014

14,062 Views

1Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University

This article describes a neuronal culture system that can be used to obtain large quantities of pure axonal samples for biochemical and immunocytochemical analyses. This technique will allow an improved understanding of normal axonal physiology and signaling pathways leading to neurodegeneration.

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