S'identifier

JoVE Journal

Bioengineering

This content is Open Access.

Isolation and Decellularization of a Whole Porcine Pancreas

9.2K Views

09:08 min

October 10th, 2018

October 10th, 2018

9,184 Views

0:04

Title

1:01

Porcine Pancreas Dissection

4:51

Decellularization Preparation

6:00

Porcine Pancreas Decellularization

7:33

Results: Representative Gross Morphology and H&E Staining of Normal and Decellularized Pancreata

8:11

Conclusion

Transcription

Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

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