qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

8.5K Views

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07:58 min

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March 6th, 2019

DOI :

10.3791/58646-v

March 6th, 2019

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Jyothi Jayaraman¹^,²^,³^,⁴, Vitalina Kirgizova¹, Da Di¹^,⁵, Christopher Johnson¹^,⁶, Wei Jiang¹^,⁷, James A. Traherne¹

¹Department of Pathology, University of Cambridge, ²Department of Physiology, Development and Neuroscience, University of Cambridge, ³Department of Obstetrics and Gynaecology, University of Cambridge School of Medicine, NIHR Cambridge Biomedical Research Centre, ⁴Centre for Trophoblast Research, University of Cambridge, ⁵Department of Genetics & Evolution, University of Geneva, ⁶Royal Papworth Hospital, ⁷Department of Plant Sciences, University of Cambridge

Summary

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QKAT

Quantitative KIR Automated Typing

Stem Cell Transplantation

High throughput Technique

Multiplex Reactions

Chapters in this video

0:04

Title

1:45

Preparation and Plating Out of DNA

2:25

Preparation of the Master Mix

3:36

qPCR Assay

4:07

Post-run Analysis

5:19

Export of Results and Copy Number Calculations

6:39

Results: qKAT Used to Copy Number Type KIR Genes

6:59

Conclusion

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