Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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8.8K Views

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13:04 min

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March 1st, 2019

DOI :

10.3791/59233-v

March 1st, 2019

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Carl Robert Rankin¹, Janet Treger¹, Emmanuelle Faure-Kumar², Jihane Benhammou¹, Deborah Anisman-Posner³, Alex Edward Bollinger³, Charalabos Pothoulakis¹, David Miguel Padua¹

¹Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, ²Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, ³Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

Podsumowanie

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Long Noncoding RNAs

Gene activating CRISPR

Isoforms

Endogenous Expression

Rozdziały w tym wideo

0:04

Title

0:40

Vector Design and Generation

1:52

Virus Generation

4:24

Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit

7:07

dCas9 and gRNA Transduction, Selection, and Clone Creation

10:18

Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA

12:08

Conclusion

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