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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

8.7K Views

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13:04 min

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March 1st, 2019

DOI :

10.3791/59233-v

March 1st, 2019

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8,688 Views
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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Keywords Long Noncoding RNAs
Gene Activation
CRISPR
Isoforms
Genome
Endogenous Expression
Guide RNA
Deactivated Cas9
Lentivirus
HEK293T Cells
Transfection
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