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Determination of Total Lipid and Lipid Classes in Marine Samples

DOI :

10.3791/62315-v

December 11th, 2021

December 11th, 2021

3,560 Views

1Department of Ocean Sciences and CREAIT Network, Memorial University of Newfoundland

This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.

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