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A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

DOI :

10.3791/63386-v

10:37 min

January 21st, 2022

January 21st, 2022

5,602 Views

1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

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