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Cytotoxicity Assays with Zebrafish Cell Lines

DOI :

10.3791/64860-v

January 6th, 2023

January 6th, 2023

4,517 Views

1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Genetics, Federal University of Paraná (UFPR)

This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.

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