Results: Calculation of Cell Viability for Different Test Chemicals
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Conclusion
Transcription
The cytotoxicity assay protocol using zebrafish cell lines can answer questions regarding acute toxicity of chemicals on fish or help define the appropriate test concentrations for other alternative fish assays. The main advantage of this 96-well
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This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.