Hepatocyte Pre-incubation with Cryoprotective Agents (CPAs)
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Bulk Droplet Vitrification
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Cryogenic Storage
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Rewarming of the Vitrified Hepatocyte Droplets
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Results: Comparison of Bulk Droplet Vitrification to Classic Cryopreservation
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Conclusion
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Vitrification is typically handled by toxicity of cryoprotective agents. And re-inserted to small samples that can be cooled fast. This protocol enables vitrification of large cell quantities while using a low CPA concentration during pre-incubati
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This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.