Hepatocyte Pre-incubation with Cryoprotective Agents (CPAs)
4:59
Bulk Droplet Vitrification
5:51
Cryogenic Storage
7:00
Rewarming of the Vitrified Hepatocyte Droplets
9:05
Results: Comparison of Bulk Droplet Vitrification to Classic Cryopreservation
10:21
Conclusion
Transkript
Vitrification is typically handled by toxicity of cryoprotective agents. And re-inserted to small samples that can be cooled fast. This protocol enables vitrification of large cell quantities while using a low CPA concentration during pre-incubati
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This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.