Results: MicroRNA Biogenesis Reporter Assay Analyzing Maturation in Mammalian Cells
6:17
Conclusion
Transcribir
Our protocol addresses the effects of regulatory RNA-binding proteins on microRNA biogenesis and maturation, straight from cell cultures. The method we describe can be performed with common cell culture reagents and is relatively fast. This method
Inicia sesión o comienza tu prueba gratuita para acceder a este contenido
We developed an intronic microRNA biogenesis reporter assay to be used in cells in vitro with four plasmids: one with intronic miRNA, one with the target, one to overexpress a regulatory protein, and one for Renilla luciferase. The miRNA was processed and could control luciferase expression by binding to the target sequence.