Extraction and Purification of Sporozoites of Eimeria spp. (e.g., E. tenella)
3:17
Collection and Purification of Merozoites of E. necatrix
4:39
Nucleofection of Merozoites or Sporozoites
5:57
Cloacal Inoculation or Intravenous Injection
6:36
Results: Meronts, Merozoites, Sporocysts, Sporozoites and Oocysts
7:20
Conclusion
Transcription
Establishment of transfection protocols for Eimeria species would help to advance the study of gene functions, developing novel vaccines, and screening new drug targets for Eimeria. Using this technique, we achieved the transfection of several spe
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Here, we provided a method to achieve stable transfection of chicken Eimeria parasites by nucleofecting sporozoites or second-generation merozoites. Genetically modified eimerian parasites expressing heterologous antigenic genes could be used as vaccine delivery vehicles.