This protocol provides reproducible, immunocompetent PDAC in vivo models suitable for genotype, phenotype, and drug response studies. The main advantage of this technique is its ability to help investigate tumor progression with a syngeneic biolog
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Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.