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JoVE Journal

Biochemistry

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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

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06:45 min

February 28th, 2019

February 28th, 2019

8,447 Views

0:04

Title

0:31

Sample Preparation

1:40

Atomic Force Microscope (AFM) Setup

3:27

AFM Calibration and Data Acquisition

5:28

Results: AFM-Based Single-Molecule Force Spectroscopy of (I91)3-NI10C-(I91)3

6:20

Conclusion

필기록

Single-molecule force spectroscopy can provide enormous insights into molecular biology on the atomic scale by allowing us to manipulate single molecules with force. Here, we demonstrate a constant velocity measurement using atomic force microscop

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We describe the detailed procedures and strategies to measure the mechanical properties and mechanical unfolding pathways of single protein molecules using an atomic force microscope. We also show representative results as a reference for selection and justification of good single protein molecule recordings.

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