Dereplication Plate MHB Overlay and ARP/MARP Library Plate Preparation
6:51
Dereplicating using the ARP/MARP
7:59
Results: Dereplication Results
9:35
Conclusion
필기록
Our protocol is significant because it allows for both the cost effective and timely dereplication of known antibiotics without the use of specialized equipment. The main advantage to this technique is template customization. This means that an in
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We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.