Rodent Intravenous Catheterization, Virus Delivery, and Optic Fiber Implantation
4:11
Rodent Cocaine Self-Administration and Instrumental Lever Extinction
5:25
In Vivo Optogenetic Induction of Long-Term Depression (LTD)
6:40
Test the Effect of Optogenetic Stimulation on Cue-Induced Cocaine Seeking
7:15
Results: The Behavioral and Histological Analysis after Cocaine Self-Administration Experiments in Rats
9:02
Conclusion
필기록
This protocol is significant because it uses novel advances in optogenetic techniques and a preclinical model of substance abuse to reverse plasticity in neural circuits that are responsible for promoting relapse. The main advantage of this techni
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
The methods described here outline a procedure used to optogenetically reverse cocaine-induced plasticity in a behaviorally-relevant circuit in rats. Sustained low-frequency optical stimulation of thalamo-amygdala synapses induces long-term depression (LTD). In vivo optogenetically-induced LTD in cocaine-experienced rats resulted in the subsequent attenuation of cue-motivated drug seeking.