Inoculation, Culturing, and Subculturing of C. albicans
1:29
Preparation of the Peptide Solutions in a 96-Well Plate
2:33
Dilution of the C. albicans Subculture
3:37
Incubation of C. albicans
5:00
Determination of Antifungal Activity
5:34
Results: Assessment of the Antifungal Activity of Histatin 5 and Variants of Histatin 5 Containing Modifications at the Lysine Residues
6:05
Conclusion
필기록
Peptides are being studied as novel antifungal molecules. This protocol can be used to study the antifungal efficacy against the human fungal pathogen Candida albicans. This technique provides quantitative data on antifungal activity while reducin
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
This protocol describes a method for obtaining quantitative data on the antifungal activity of peptides and other compounds, such as small-molecule antifungal agents, against Candida albicans. Its use of optical density rather than counting colony-forming units to quantify growth inhibition saves time and resources.