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JoVE Journal

Immunology and Infection

This content is Free Access.

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

2.4K Views

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10:33 min

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December 17th, 2021

December 17th, 2021

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2,427 Views

0:05

Introduction

1:26

Antigen Preparation for Subcutaneous Injection

2:31

Subcutaneous Injection

3:31

Intravitreal Injection Procedure

5:57

Optical Coherence Tomography (OCT) Imaging to Detect and Quantify Uveitis

7:35

Results: Primed Mycobacterial Uveitis Model for Induction of Uveitis in Mice

9:12

Conclusion

Transcrição

This protocol outlines the steps for inducing Primed Mycobacterial Uveitis (PMU) in mice. This method outlines the steps to help produce reliable and robust ocular inflammation in the mouse model system. Using this protocol, we generated uveitic eyes and uninflamed fellow eyes from single animals for further evaluation with immunologic, transcriptomic, and proteomic assays.

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