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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

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04:45 min

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February 10th, 2022

DOI :

10.3791/60607-v

February 10th, 2022

•

6,328 Views

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*¹^,³^,⁴, Emad Tajkhorshid¹^,³^,⁴, Rutilio A. Fratti¹^,³

¹Department of Biochemistry, University of Illinois at Urbana-Champaign, ²Department of Chemistry, University of South Florida, ³Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, ⁴Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign

Chapitres

Transcription

Résumé

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Microscale Thermophoresis

Protein lipid Interactions

Vam7

Phosphatidic Acid

Disassociation Constant KD

Biochemical Interactions

NTA Atto 647 Dye

Thermophoretic Traces

1 1 Titration

DiC8 PA

Pharmaceutical Development

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