Collection of Pupae for Microscopic Characterization and Identification of Fluorescent Markers
1:45
Sexing Pupae
2:25
Setting Up GAL4-UAS (Upstream Activation Sequences) System Crosses and Inducing Egg Laying
3:20
Embryo Fixation and Bleaching
4:15
Results: Analysis of Anopheles gambiae Pupae Expressing Fluorescent Markers Driven by 3xP3 Promoter, Tissue-specific Expression Visualization, and Embryo Clearing
5:53
Conclusion
Transkript
The bipartite GAL4-UAS system is a versatile tool for functional genetic analysis in Anopheles gambiae through genetic modification of gene expression in a spatiotemporal controlled manner. The binary system enables flexibility in experimental app
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The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.