Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

5.8K Views

06:29 min

April 15th, 2021

DOI :

10.3791/62131-v

April 15th, 2021


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GAL4 UAS System

Chapters in this video

0:05

Introduction

0:49

Collection of Pupae for Microscopic Characterization and Identification of Fluorescent Markers

1:45

Sexing Pupae

2:25

Setting Up GAL4-UAS (Upstream Activation Sequences) System Crosses and Inducing Egg Laying

3:20

Embryo Fixation and Bleaching

4:15

Results: Analysis of Anopheles gambiae Pupae Expressing Fluorescent Markers Driven by 3xP3 Promoter, Tissue-specific Expression Visualization, and Embryo Clearing

5:53

Conclusion

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