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Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

DOI :

10.3791/62131-v

April 15th, 2021

April 15th, 2021

4,528 Views

1Department of Vector Biology, Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology, University of Crete

The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.

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