Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
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09:56 min
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April 30th, 2019
DOI :
April 30th, 2019
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Chapters in this video
0:04
Title
1:28
Preparing the Equipment for the Microinjection
2:21
Preparing Embryos for Transplantation
2:56
Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
4:56
Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
6:29
Injecting Mixed Cell Suspension into the Zebrafish
7:10
Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
8:11
Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
9:12
Conclusion
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