Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

4.6K Views

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07:02 min

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April 15th, 2022

DOI :

10.3791/63875-v

April 15th, 2022

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Rhiannon B. Werder*¹^,²^,³, Jessie Huang*¹^,², Kristine M. Abo¹^,², Olivia T. Hix¹^,², Kasey Minakin¹^,², Konstantinos-Dionysios Alysandratos¹^,², Carly Merritt¹^,², Kayleigh Berthiaume¹^,², Andrea B. Alber¹^,², Claire L. Burgess¹^,², Darrell N. Kotton*¹^,², Andrew A. Wilson*¹^,²

¹Center for Regenerative Medicine, Boston University and Boston Medical Center, ²The Pulmonary Center and Department of Medicine, Boston University School of Medicine, ³QIMR Berghofer Medical Research Institute

Summary

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3D Spheres

2D Air liquid Interface

Human Induced Pluripotent Stem Cells

Type 2 Alveolar Epithelial Cells

Lung Biology

Pulmonary Diseases

Alveolospheres

Directed Differentiation

Iscove s Modified Dulbecco s Medium

Environmental Exposure

Chapters in this video

0:04

Introduction

0:57

Alveolosphere Dissociation

2:33

3D Plating of iAT2s

3:37

Passaging of iAT2s to ALI

5:22

Results: Human Induced Pluripotent StemCell-Derived Type 2 Alveolar Epithelial-Like Cells

6:05

Conclusion

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