Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

1.0K Views

07:31 min

February 17th, 2023

DOI :

10.3791/64977-v

February 17th, 2023


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PAM Less Base Editing

Chapters in this video

0:04

Introduction

1:02

Preparation of Microinjection Capillaries and Modified RNA

2:23

RNA Microinjection of Zebrafish Embryos

4:36

Efficiency Analysis of EditR Base Edits

6:08

Results: TSR2 Diamond Blackfan Zebrafish Mutation

6:41

Conclusion

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