Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

2.2K Views

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14:54 min

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September 11th, 2023

DOI :

10.3791/65804-v

September 11th, 2023

•

Julian Wolf¹^,², Teja Chemudupati¹^,², Aarushi Kumar¹^,², Ditte K. Rasmussen¹^,², Karen M. Wai², Robert T. Chang², Artis A. Montague², Peter H. Tang³^,⁴, Alexander G. Bassuk⁵^,⁶^,⁷, Antoine Dufour⁸^,⁹, Prithvi Mruthrunjaya², Vinit B. Mahajan¹^,²^,¹⁰

¹Molecular Surgery Laboratory, Stanford University, ²Department of Ophthalmology, Byers Eye Institute, Stanford University, ³Department of Ophthalmology and Visual Neurosciences, University of Minnesota, ⁴Retina Consultants of Minnesota, ⁵Department of Pediatrics, University of Iowa, ⁶Department of Neurology, University of Iowa, ⁷The Iowa Neuroscience Institute (INI), University of Iowa, ⁸Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, ⁹Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, ¹⁰Veterans Affairs Palo Alto Health Care System

Summary

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Biobanking

Human Aqueous Humor

Vitreous Liquid Biopsies

Molecular Profiling

Ocular Diseases

Diagnostic Strategies

Therapeutic Strategies

Intraocular Surgery

Data Privacy Database

Anterior Chamber Paracentesis

Surgical Protocol

Vitreoretinal Surgeon

Chapters in this video

0:12

Introduction

1:08

Collection of aqueous humor liquid biopsies

2:54

mCollection of vitreous liquid biopsies

6:56

Transferring cryovials to storage

8:10

Sample storage form

12:10

Representative Results

14:19

Conclusions

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