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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

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10:11 min

July 26th, 2024

DOI :

10.3791/67076-v

July 26th, 2024


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Hybrid Ensemble Assay

Chapters in this video

0:00

Introduction

1:41

Binding of Doubly Functionalized Linear DNA Substrate to Streptavidin‐Coated Magnetic Beads

3:59

Ensemble Assembly and Activation of Fluorescently Labeled CMG onto Magnetic Bead‐Bound DNA for Single Molecule Imaging

7:16

Single‐Molecule Imaging of Fully Reconstituted CMG with Correlative Dual‐Beam Optical Tweezers and Confocal Microscopy

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