Cytosolic and Nuclear Protein Fraction Preparation
3:52
Labeling RNA with Desthiobiotin
6:04
RNA-Protein Pulldown
9:24
Results: High Specificity Binding Via Protocol
10:33
Conclusion
필기록
The overall goal of this procedure is to capture cytosolic protein that bind to a specific sequence located in RNA molecule in mesothelioma cells. This method can help answer key question in RNA biology field, such as if a predicted RNA binding pr
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Desthiobiotin labeling of a synthetic 25-nucleotide RNA oligo, which contains an adenine-rich element (ARE) motif, allows specific binding of cytosolic ARE-binding protein.