This is a novel method for the assessment of the glucocorticoid hormone, cortisol, from koala fur. It provides a novel biomarker for the assessment of chronic stress in koalas. The main advantage of this technique is that it is a highly sensitive
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We present a protocol to determine the optimal extraction solvent to measure cortisol from koala fur. The solvents used in this protocol are methanol, ethanol and isopropanol. Determining an optimal extraction solvent will aid in reliably measuring fur to determine the impact of chronic stress on koalas.