Immunostaining without Membrane Permeabilization to Stain Rhodopsin Protein on the Cell Surface
4:43
Imaging
7:12
Image Analysis
9:12
Results: Characterization of Rhodopsin Transport
10:50
Conclusion
Transkript
Rhodopsin misfolding causes progressive retinal degeneration called retinitis pigmentosa. While a traditional imaging method have limited capacity to quantify the rhodopsin transport, this newly-developed image-based assay allows for high throughp
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Here, we described a high-content imaging method to quantify the transport of rhodopsin mutants associated with retinitis pigmentosa. A multiple-wavelength scoring analysis was used to quantify rhodopsin protein on the cell surface or in the whole cell.