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A Rhodopsin Transport Assay by High-Content Imaging Analysis

DOI :

10.3791/58703-v

January 16th, 2019

January 16th, 2019

6,349 Views

1Department of Ophthalmology, University of Pittsburgh, 2McGowan Institute for Regenerative Medicine, University of Pittsburgh

Here, we described a high-content imaging method to quantify the transport of rhodopsin mutants associated with retinitis pigmentosa. A multiple-wavelength scoring analysis was used to quantify rhodopsin protein on the cell surface or in the whole cell.

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Rhodopsin Transport Assay

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