Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
11.9K Views
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08:12 min
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March 11th, 2020
DOI :
March 11th, 2020
•Yaolei Mi1,2, Zhihui Zhu3, Guangtao Qian3, Yu Li4, Xiangxiao Meng1, Jianping Xue3, Qingfu Chen5, Wei Sun1, Yuhua Shi1,2
1Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 2Artemisinin Research Center, China Academy of Chinese Medical Sciences, 3College of Life Science, Huaibei Normal University, 4Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, 5Research Center of Buckwheat Industry Technology, Guizhou Normal University
Chapters in this video
0:04
Introduction
1:01
Tartary Buckwheat (TB) Seed Preparation
1:49
TB Seedling and Explant Preparation
2:46
A. rhizogenes Transformation Preparation
3:56
TB Explant Screening, Infection, and Co-Culture
4:47
Induction and Selective Culture and TB Hairy Root Subculture
5:32
Transformed Hairy Root Identification and Conservation
6:14
Results: Representative Hairy Root Transformation Identification and Characterization
7:23
Conclusion
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