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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

11.8K Views

08:12 min

March 11th, 2020

DOI :

10.3791/60828-v

March 11th, 2020


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Agrobacterium Rhizogenes

Chapters in this video

0:04

Introduction

1:01

Tartary Buckwheat (TB) Seed Preparation

1:49

TB Seedling and Explant Preparation

2:46

A. rhizogenes Transformation Preparation

3:56

TB Explant Screening, Infection, and Co-Culture

4:47

Induction and Selective Culture and TB Hairy Root Subculture

5:32

Transformed Hairy Root Identification and Conservation

6:14

Results: Representative Hairy Root Transformation Identification and Characterization

7:23

Conclusion

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