1.3K Views
•
08:02 min
•
March 3rd, 2023
DOI :
March 3rd, 2023
•Chapters in this video
0:05
Introduction
0:43
Cell Culture and Harvesting
1:30
Microtubule Extraction
4:13
Cryo-Electron Microscopy (EM) Grid Preparation
6:16
Results: Analyzing Microtubule-Protein Complexes
7:34
Conclusion
Related Videos
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
14.8K Views
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
14.8K Views
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
9.4K Views
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
3.9K Views
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
3.2K Views
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
4.1K Views
Single Particle Cryo-Electron Microscopy: From Sample to Structure
8.2K Views
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
6.4K Views
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
2.5K Views
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
12.6K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved