L. monocytogenes-infected Mouse Dissection and Cardiac Perfusion
1:51
Organ Harvest and Bacterial Burden Determination
4:00
Results: Representative Bacterial Burdens in Infected Mouse Organs
4:22
Conclusion
Transcription
This method can help answer key questions in the bacterial pathogenesis field about whether bloodborne bacteria can cross the blood-brain barrier to infect the central nervous system and brain. With this technique, bacterial numbers within the blo
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During infection, Listeria monocytogenes is capable of crossing the blood-brain barrier to colonize the brain. In this protocol, we demonstrate how to assess bacterial colonization of organs following infection of mice. A procedure to perform whole organ perfusion for specific determination of bacterial numbers in the brain parenchyma is provided.