April 30th, 2020
•Repeat-associated non-ATG-dependent translational products are emerging pathogenic features of several repeat expansion-based diseases. The goal of the protocol described is to evaluate toxicity caused by these peptides using behavioral and cellular assays in the model system C. elegans.
Vidéos Connexes
Generation of Stable Transgenic C. elegans Using Microinjection Video (Video) | JoVE
Generation of Transgenic C. elegans by Biolistic Transformation Video (Video) | JoVE
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans Video (Video) | JoVE
Measuring Peptide Translocation into Large Unilamellar Vesicles Video (Video) | JoVE
Basic Caenorhabditis elegans Methods: Synchronization and Observation Video (Video) | JoVE
C. elegans Chemotaxis Assay Video (Video) | JoVE
Antibody Staining in C. Elegans Using "Freeze-Cracking" Video (Video) | JoVE
Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay Video (Video) | JoVE
An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans Video (Video) | JoVE
Methods for Skin Wounding and Assays for Wound Responses in C. elegans Video (Video) | JoVE