Purification of Ubiquitinated Proteins under Nondenaturing Conditions
5:01
Purification of Ubiquitinated Proteins under Denaturing Conditions
7:49
Detection of Purified Ubiquitinated Proteins by Western Blotting
8:29
Results: Ubiquitinated p53 Proteins Purified under Nondenaturing and Denaturing Conditions
10:22
Conclusion
Transcription
Using this protocol, the ubiquitinated forms of a given protein can be efficiently purified from mammalian cells, facilitating studies on the roles of ubiquitination in regulating protein function. Purification of ubiquitinated proteins in mammali
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The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.