The aim of this manuscript was to evaluate the suitability of the simultaneous quantification of Gamma H2AX and 53BP1 foci to assess the genomic damage induced in human peripheral lymphocytes by bleomycin. Several factors can cause double strand b
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This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.